Species:

Mouse

Alternative Name:

CD295, LEP-R, LR, LEPRD, OB-R, OBR

Uniport ID:

P48356

Assay Type:

Sandwich

unit:

ng/mL

Standard:

200ng/mL

Sensitivity:

1.88 ng/mL

Detection Range:

3.13-200ng/mL

Assay Time:

3H

Sample Type:

Serum, Plasma and Tissue homogenate and Other biological samples；Sample volume：25μL

Research:

Signaling transduction, Cancer, Metabolism

Test Principle:

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse LEPR. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody and biotinylated detection antibody specific for Mouse LEPR. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse LEPR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Mouse LEPR. You can calculate the concentration of Mouse LEPR in the samples by comparing the OD of the samples to the standard curve.